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Updated: Aug 8, 2026
Real-Time High Throughput Technique to Quantify Neutrophil Extracellular Traps Formation in Human Neutrophils
Published on: December 1, 2023
Protocol to investigate the role of NETosis in health and disease via a high-throughput neutrophil-like model using
Gavin Li1, Sara Versales1, Brett Guadagnino1
1Department of Human Biology and Genomic Medicine, J. Craig Venter Institute, La Jolla, CA 92037, USA.
Abstract:
Neutrophil extracellular traps (NETs) are a crucial host-defense mechanism employed by neutrophils in response to various aggregates and contribute to pathology in diverse diseases, from chronic inflammation to sepsis. Here, we present a protocol to investigate the role of NETosis, the release of NETs, in health and disease via a high-throughput neutrophil-like model using quantitative imaging. We describe steps for preparing stimulated, differentiated Human Leukemia-60 (HL-60) cells; immunostaining; high-throughput microscopy; and NETosis quantification using CellProfiler.
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