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Protocol for tumor detection in mice using β-glucuronidase targeted breath analysis
Guochao Wu1, Tianran Si1, Han Jin2
1Institute of Nano Biomedicine and Engineering & National Key Laboratory of Advanced Micro and Nano Manufacture Technology, School of Automation and Intelligent Sensing, Shanghai Jiao Tong University, Shanghai 200240, P.R. China.
STAR Protocols
|August 6, 2026
Summary
This study introduces a novel breath analysis method for early tumor detection in mice. The technique uses phenyl-β-D-glucuronide to target β-glucuronidase, enabling sensitive identification of tumors.
Area of Science:
- Biomedical Engineering
- Oncology
- Analytical Chemistry
Background:
- Early tumor detection significantly improves patient cure rates.
- The tumor microenvironment often exhibits unique biochemical markers.
- Non-invasive diagnostic strategies are highly sought after in cancer research.
Purpose of the Study:
- To present a protocol for early tumor detection in mice using a targeted breath analysis strategy.
- To detail the development and application of a novel breath analysis device for cancer diagnostics.
- To establish a sensitive method for identifying tumors based on exhaled breath biomarkers.
Main Methods:
- Development of a targeted breath-analysis strategy using phenyl-β-D-glucuronide.
- Specific targeting of the enzyme β-glucuronidase, which is overexpressed in tumors.
- Protocol includes drug screening, enzymatic reaction analysis, stability and toxicity evaluation.
- Construction and utilization of a specialized mouse exhaled-breath extraction device.
- Detailed procedures for animal-model breath analysis.
Main Results:
- Demonstrated a highly sensitive method for early tumor detection in a mouse model.
- Validated the targeted breath-analysis strategy for identifying tumor-specific biomarkers.
- The protocol enables non-invasive monitoring and detection of tumors.
Conclusions:
- The described breath-analysis protocol offers a promising tool for sensitive and early tumor detection in preclinical models.
- This targeted approach, focusing on β-glucuronidase activity, represents a significant advancement in non-invasive cancer diagnostics.
- Further research can explore the translation of this technology for human clinical applications.
