Investigation of the miRNA27a rs895819, miRNA222 rs2858060 and miRNA499a rs3746444 polymorphisms in Endometriosis

The present study aimed to determine the allelic and genotypic frequencies of the miRNA27a rs895819, miRNA222 rs2858060 and miRNA499 rs3746444 polymorphisms and their possible association with endometriosis. This case-control study consisted of 143 women; 54 had endometriosis (case group) and 89 had no endometriosis (control group) ant the polymorphisms were determined by real-time PCR. For the statistical analysis, the chi-square was used to compare genotypic and allelic frequencies, estimate the Hardy-Weinberg equilibrium, and logistic regression test. There were no significant differences in genotype frequencies between case and control of miRNA27a rs895819 (X² = 2.57; p = 0.27) and of miRNA499a rs3746444 (χ2 = 3.65; p = 0.16), in other hand, exist a difference (X² = 8.42; p = 0.01) for miRNA222 rs2858060. Patients and controls were in Hardy-Weinberg equilibrium to rs895819, however to the polymorphisms rs2858060 and rs 3746444 were in disequilibrium to the controls. In the sample analyzed, the rs895819 polymorphism of miRNA-27a and the rs3746444 miRNA-499a are not associated with endometriosis, while the miRNA-222 rs2858060 displays a preliminary and exploratory indication of association. This work represents an advance in endometriosis genetics since it reveals a link between endometriosis and miRNA variants.