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Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides
Published on: June 19, 2012
SsrA-Based Targeted Protein Degradation in Escherichia coli
Qichang Nie1, Terrence Chi-Kong Lau2
1Department of Biomedical Sciences, College of Biomedicine, City University of Hong Kong, Kowloon, Hong Kong, SAR, China.
None:
Targeted protein degradation is an emerging concept of drug discovery to selectively eliminate the pathogenic proteins by activating their degradation in cells. The proteolysis-targeting chimeras (PROTACs) are bifunctional small molecules that induce the degradation of a protein of interest (POI) by proteasome. Here we describe a method that utilizes an ssrA-based BacPROTAC (bacterial PROTACs) to target the drug-resistant proteins CTX-M-14, a class A β-lactamase commonly found in extended-spectrum beta-lactamase (ESBL) plasmids of antimicrobial resistance (AMR) bacteria. This strategy could resensitize the drug-resistant bacteria and revive previously disregarded antibiotics, opens up a new avenue for therapeutic development of AMR bacteria.
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