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Updated: Aug 13, 2026
Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone
Published on: April 8, 2015
Protocol for isotype-resolved 3D imaging and quantification of bone marrow plasma cells in murine femurs
Abdouramane Camara1, Baizhi Chen2, An Qi Xu2
1Immunity and Cancer Laboratory, The Francis Crick Institute, London, UK; West African Centre for Cell Biology of Infectious Pathogens, University of Ghana, Accra, Ghana.
Abstract:
Bone marrow plasma cells maintain durable antibody responses, but their tissue localization and morphology are difficult to quantify in situ. Here, we present a protocol for isotype-resolved 3D imaging and quantification of bone marrow plasma cells in murine femurs. We describe steps for fixation, optimal cutting temperature (OCT) coumpound embedding, longitudinal opening of mouse femurs, whole-mount immunostaining, optical clearing, and confocal 2D/3D imaging. We detail an Imaris-based workflow for reproducible single-cell segmentation, isotype-resolved counting, and the extraction of morphological features from defined regions of interest.
