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Identification of Clinically Relevant Hb Variants During HbA1c Testing Using the ARKRAY HA-8180V in Fast Mode:
Monthon Mayoon1, Sitthichai Panyasai2
1Department of Medical Technology, Lampang Hospital, Lampang, Thailand.
Annals of Laboratory Medicine
|August 12, 2026
Summary
The ARKRAY HA-8180V HPLC system accurately identifies hemoglobin E (HbE) and other variants during HbA1c testing. This method prevents misinterpretation of HbA1c results caused by common hemoglobin variants.
Area of Science:
- Clinical Chemistry
- Hematology
- Analytical Biochemistry
Background:
- Hemoglobin (Hb) variants are frequently discovered incidentally during HbA1c testing.
- These variants can interfere with the accuracy of HbA1c quantification.
Purpose of the Study:
- To evaluate the ARKRAY HA-8180V HPLC system's fast mode (FM) for identifying Hb variants, especially HbE.
- To assess the system's ability to use characteristic retention times (RTs) for variant detection in samples undergoing HbA1c testing.
Main Methods:
- Analysis of 4,888 HbA1c chromatograms for abnormal patterns.
- Further evaluation of abnormal samples using capillary electrophoresis (CE) and globin gene sequencing.
- Recording of retention times (RTs) for variant identification.
Main Results:
- 12.0% of chromatograms were abnormal; 95.9% of these showed peaks consistent with HbE.
- Identified Hb phenotypes included HbE (EA, EE), compound heterozygotes, and other variants like Hb Hope and Hb Korle-Bu.
- Hb Hope and Hb CSA2ABart'sH variants interfered with HbA1c measurements, causing falsely low values in some cases.
Conclusions:
- Hemoglobin E and thalassemia variants can significantly interfere with HbA1c quantification.
- Review of ARKRAY HA-8180V FM chromatograms reliably detects HbE and prevents HbA1c result misinterpretation.
