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Updated: Aug 13, 2026
06:33
Production of Human CRISPR-Engineered CAR-T Cells
Published on: March 15, 2021
Rewriting CAR-T cell fate: CRISPR/Cas gene editing for solid tumor therapy
Wenjing Liu1, Jiayi Gu1, Chenghao Xie1
1Shanghai Frontiers Science Center of Genome Editing and Cell Therapy, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, China.
Frontiers in Immunology
|August 12, 2026
Summary
CRISPR/Cas gene editing enhances chimeric antigen receptor T (CAR-T) cell therapy for solid tumors by overcoming challenges like T cell exhaustion and immunosuppression. This review explores strategies to improve CAR-T efficacy in solid tumors.
Area of Science:
- Oncology
- Immunotherapy
- Gene Editing Technology
Background:
- Chimeric antigen receptor T (CAR-T) cell therapy shows success in blood cancers but faces limitations in solid tumors.
- Key challenges include poor tumor infiltration, T cell exhaustion, and an immunosuppressive tumor microenvironment (TME).
Purpose of the Study:
- To review recent advancements in applying CRISPR/Cas gene editing to enhance CAR-T cell therapy for solid tumors.
- To discuss current challenges and propose strategies for improving CAR-T efficacy against solid tumors.
Main Methods:
- Literature review of CRISPR/Cas applications in CAR-T cell therapy for solid tumors.
- Analysis of strategies to overcome TME-related barriers and T cell dysfunction.
Main Results:
- CRISPR/Cas technology offers a powerful tool to engineer CAR-T cells for improved antitumor activity.
- Gene editing can address issues like tumor infiltration and T cell exhaustion.
Conclusions:
- CRISPR/Cas-mediated CAR-T cell engineering holds significant promise for treating solid tumors.
- Further research and strategic implementation are crucial to overcome existing limitations and maximize therapeutic potential.
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