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Updated: Aug 14, 2026
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Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice
Published on: January 25, 2021
QKI Promotes Sheep Preadipocyte Differentiation by Reducing Cavin3 mRNA Stability
Zicheng Li1,2, Changsong Xu1,2, Bokang Shan1,2
1College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China.
Animals : an Open Access Journal From MDPI
|August 13, 2026
Summary
Quaking (QKI) promotes sheep preadipocyte differentiation by stabilizing Cavin3 mRNA. This RNA-binding protein regulates adipogenesis, impacting fat deposition and metabolic health in livestock.
Area of Science:
- Molecular Biology
- Animal Science
- Genetics
Background:
- Adipogenic differentiation is crucial for livestock metabolic regulation and fat deposition.
- The role of RNA-binding proteins, like Quaking (QKI), in sheep preadipocyte differentiation is not well understood.
Purpose of the Study:
- To investigate the role of Quaking (QKI) in regulating sheep preadipocyte adipogenesis.
- To explore the downstream regulatory mechanisms of QKI in this process.
Main Methods:
- Examined QKI expression in sheep adipose tissues.
- Performed QKI knockdown in sheep preadipocytes.
- Utilized full-length transcriptome sequencing (ONT), RNA immunoprecipitation coupled with quantitative real-time PCR (RIP-qPCR), and actinomycin D assays.
- Assessed adipogenic marker expression, lipid accumulation (Oil Red O staining), and glucose consumption.
Main Results:
- QKI knockdown reduced adipogenic marker expression, lipid accumulation, and glucose consumption.
- Transcriptome sequencing identified Cavin3 as a downstream target of QKI.
- RIP-qPCR and actinomycin D assays confirmed QKI binds to Cavin3 mRNA and stabilizes it.
- Cavin3 knockdown enhanced adipogenesis, while simultaneous QKI and Cavin3 knockdown partially rescued adipogenic marker expression.
Conclusions:
- QKI promotes sheep preadipocyte differentiation by decreasing Cavin3 mRNA stability.
- This study provides insights into RNA-binding protein regulation of adipogenesis in livestock.
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