Magnesium-Containing Calcium Silicate Sealer Modulates Bioactive Gene Expression in iPDLCs
Soraya Viana Gadelha1, Ana Carolina Bontempi1, Natália Bispo de Sousa1
1Health Science Institute, Dental Research Division, Paulista University, Rua Doutor Bacelar, 1212. 04026-002, Sao Paulo, Brazil.
Journal of Endodontics
|August 13, 2026
Summary
Bio-C Sealer Ion+ demonstrated adequate cytocompatibility and enhanced gene expression of proliferation and osteogenic markers in human periodontal ligament stem cells (iPDLCs), suggesting improved repair potential.
Area of Science:
- Biomaterials Science
- Dental Materials Science
- Regenerative Dentistry
Background:
- Calcium silicate-based endodontic sealers are crucial for root canal therapy.
- Evaluating the cytocompatibility and bioactivity of novel bioceramic sealers is essential.
- Understanding cellular responses to endodontic materials informs clinical application.
Purpose of the Study:
- To compare the cytocompatibility and transcriptional bioactivity of a magnesium-containing calcium silicate sealer (Bio-C Sealer Ion+) with existing bioactive sealers.
- To assess the impact of these sealers on immortalized human periodontal ligament stem cells (iPDLCs).
- To evaluate effects on proliferation and osteogenic marker gene expression.
Main Methods:
- Specimens of Bio-C Sealer, Bio-C Sealer Ion+, and BioRoot RCS were prepared and extracted.
- iPDLCs were exposed to material extracts.
- Mitochondrial metabolism (MTT assay) and gene expression (qRT-PCR) of specific markers were analyzed.
Main Results:
- All sealers showed adequate cytocompatibility.
- Bio-C Sealer Ion+ exhibited increased expression of proliferation markers (MAPK14, YAP1, CREB1) at 10 days.
- Bio-C Sealer Ion+ significantly upregulated osteogenic markers (COL1A1, ALPL) and showed high RUNX2 and SP7 expression.
Conclusions:
- All tested bioceramic sealers are cytocompatible with iPDLCs.
- Bio-C Sealer Ion+ demonstrates a potential to stimulate periodontal cell responses related to tissue repair.
- The magnesium-containing sealer shows promising bioactivity for endodontic applications.
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