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AI-enhanced 3D-OCT analysis for automated mapping of layer thickness in paintings
Optics Express
|August 14, 2026
Summary
This study introduces an automated workflow for analyzing 3D Optical Coherence Tomography (OCT) data. The AI-powered approach enables rapid, reproducible, and accurate measurement of paint layer thickness in art diagnostics.
Area of Science:
- Art diagnostics
- Non-invasive imaging techniques
- Cultural heritage research
Background:
- Characterizing heterogeneous paint layer stratigraphy is crucial in art diagnostics.
- Optically opaque materials impede light penetration, challenging non-invasive analysis.
- Optical coherence tomography (OCT) is a valuable non-invasive tool, but manual analysis is limited.
Purpose of the Study:
- To develop an automated workflow for 3D OCT analysis of paint layers.
- To enable rapid, reproducible, and statistically robust layer thickness quantification.
- To overcome the limitations of manual OCT data analysis in art diagnostics.
Main Methods:
- Utilized supervised semantic segmentation with a Random Forest classifier (Trainable Weka Segmentation).
- Developed a custom MATLAB script for automated interfacial distance calculation.
- Applied the workflow to 3D OCT B-scans for pixel-wise thickness measurements.
Main Results:
- Achieved rapid and fully reproducible pixel-wise thickness measurements from 3D OCT data.
- Demonstrated superior performance compared to manual analysis methods.
- Significantly reduced data processing time for OCT volume analysis.
Conclusions:
- The presented AI-based framework automates OCT segmentation and thickness quantification for cultural heritage research.
- This automated approach offers a significant advancement over traditional manual analysis.
- Enables more efficient and reliable non-invasive characterization of art materials.

