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Related Experiment Videos

Colorimetric microdetermination of plasma lactate.

J MacQueen, D Plaut

    The American Journal of Medical Technology
    |January 1, 1979
    PubMed
    Summary

    A new, rapid colorimetric method for measuring plasma lactate was developed. This simple test offers accurate and reliable results comparable to existing methods.

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    Area of Science:

    • Clinical Chemistry
    • Biochemical Assays
    • Analytical Methods

    Background:

    • Plasma lactate is a critical biomarker in various clinical conditions.
    • Existing lactate measurement methods can be time-consuming or require specialized equipment.

    Purpose of the Study:

    • To describe a novel, simple, and rapid colorimetric assay for plasma lactate.
    • To validate the accuracy and reliability of this new colorimetric procedure.

    Main Methods:

    • Development of a ten-minute colorimetric assay for plasma lactate.
    • Comparison with a standard ultraviolet (UV) spectrophotometric method.
    • Evaluation of assay precision, recovery, and potential interferences.

    Main Results:

    • The colorimetric procedure demonstrated good agreement with the established UV method.
    • Assay precision, recovery, and interference studies confirmed the method's validity.
    • The assay is suitable for routine clinical laboratory use.

    Conclusions:

    • A simple, rapid, and accurate colorimetric method for plasma lactate determination has been established.
    • This new assay provides a viable alternative to existing methods, offering efficiency and reliability.
    • The method's performance characteristics support its clinical utility.

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