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Updated: Aug 21, 2026
Determination of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice.
Published on: July 14, 2017
Identification and Evaluation of CMP-Sialic Acids
Di Wu1,2, Takayuki Omoto1, Chihiro Sato1,3
1Integrated Glyco-Biomedical Research Center (iGMED), Institute for Glyco-core Research (iGCORE), Nagoya University, Nagoya, Aichi, Japan.
None:
Cytidine 5'-monophosphate-sialic acid (CMP-Sia) is a donor substrate of all sialyltransferases in both prokaryotes and eukaryotes, and biosynthesized by the CMP-Sia synthetase (CSS or CMAS) using Sia and cytidine 5'-triphosphate (CTP) as the substrates. Since CSSs have unique substrate specificity for Sia species depending on organisms, cells, and organs, and developmental stages, various kinds of CMP-Sia exist in nature. This chapter describes how to quantitatively detect CMP-Sia in the CSS reaction mixture as well as in cells and organs.
