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Ganglioside Extraction, Purification and Profiling
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Flow Cytometry Analysis of Gangliosides Expressed on Extracellular Vesicles

Yuhsuke Ohmi1,2, Hiroka Koyanagi3, Mohammad Abul Hasnat3,4

  • 1Department of Biomedical Sciences, Chubu University Graduate School of Life and Health Sciences, Kasugai, Aichi, Japan. ooumi82@fsc.chubu.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)
|August 19, 2026
PubMed
Abstract

Keywords:
Extracellular vesiclesFlow cytometryGD2GD3GangliosideTim4

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Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...