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Ratiometric fluorescence detection of tetramethylpyrazine in complex matrices using a Tb-EDTA probe based on LMCT
Haiyan Cao1, Bin Yang1, Wenfei Dong1
1Chongqing Key Laboratory for New Chemical Materials of Shale Gas, College of Chemistry and Chemical Engineering, Yangtze Normal University, Chongqing, 408100, China.
Background:
The accurate detection of 2,3,5,6-tetramethylpyrazine (TMP) in complex matrices is significant for food quality control and biomedical analysis. Despite this need, a dedicated optical sensing platform for TMP has not been reported to date.
Results:
Here, we present a novel ratiometric fluorescent probe based on a Tb-EDTA complex, which introduces a sensing strategy founded on coordination-induced modulation of the ligand-to-metal charge transfer (LMCT) process. Specific coordination of TMP's pyrazine nitrogen atoms to the Tb3+ center results in a characteristic ratiometric response: quenching of the emission at 548 nm and concomitant enhancement at 650 nm. The probe exhibits excellent analytical performance with a linear range of 0.1-40 μM, a detection limit of 0.038 μM, and high selectivity over structurally similar interferents. Its practical applicability is demonstrated by reliable quantification of TMP in real samples, including sauce-flavor Baijiu, sake, and human urine, with recoveries of 94.54-104.82% and relative standard deviations below 10.57%.
Significance:
This work establishes a pioneering optical sensing approach for TMP detection, providing a valuable analytical tool for flavor assessment in alcoholic beverages and pharmacological monitoring in biological systems.
