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Related Experiment Videos

Competence for transfection in Staphylococcus aureus.

J E Sjöström, M Lindberg, L Philipson

    Journal of Bacteriology
    |February 1, 1973
    PubMed
    Summary
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    Lysogenicity with phage P11 is essential for Staphylococcus aureus to become competent for DNA uptake in the presence of calcium ions. This phage is crucial for natural transformation and transfection efficiency.

    Area of Science:

    • Microbiology
    • Bacteriology
    • Bacterial Genetics

    Background:

    • Staphylococcus aureus strain 8325N is naturally competent in the presence of calcium ions.
    • Competence, the ability to take up exogenous DNA, is a critical genetic process in bacteria.
    • Lysogeny, the integration of a bacteriophage into the bacterial genome, can influence bacterial traits.

    Purpose of the Study:

    • To investigate the role of bacteriophage P11 lysogeny in Staphylococcus aureus competence.
    • To determine the factors influencing DNA uptake and transfection in S. aureus.
    • To compare competence development in wild-type and mutant strains.

    Main Methods:

    • Comparative analysis of competent and non-competent Staphylococcus aureus strains (8325N, 8325-4).

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  • Use of bacteriophage P11, P12, and P13, and anti-P11 serum.
  • Induction of competence using culture filtrates and assessment of DNA uptake under varying conditions (Ca2+, pH, temperature).
  • Evaluation of energy metabolism and protein synthesis inhibition on DNA binding and uptake.
  • Comparison of competence development in wild-type and nuclease-deficient (nuc) strains.

    • Main Results:

    • Lysogenicity with phage P11 is a prerequisite for competence in Staphylococcus aureus 8325N with calcium ions.
    • Nonlysogenic derivatives (8325-4) and strains lysogenized with P12 or P13 did not develop competence.
    • Competence could be induced in non-competent cells by culture filtrates, and neutralized by anti-P11 serum.
    • Superinfection with phage P11 enhanced transfection frequency.
    • DNA uptake is sensitive to calcium ion concentration, pH, temperature, energy metabolism, and protein synthesis.
    • Wild-type strain showed a narrow competence maximum in early exponential phase, while the nuclease-deficient mutant exhibited additional maxima later.

    Conclusions:

    • Phage P11 lysogeny is essential for natural competence in Staphylococcus aureus 8325N.
    • Phage P11 or its products are directly involved in the competence mechanism.
    • Environmental factors and bacterial physiology significantly impact DNA transformation efficiency.