Intraoral cannulation for controlled taste solution delivery in mice

Presentation of taste solutions is required for investigating the detection and processing of gustatory information. These stimuli are also widely used to motivate animals in behavioral tasks. Here, we present a protocol for surgically implanting an intraoral cannula in mice, enabling direct and precise delivery of fluids for passive stimulation of oral sensory systems (e.g., gustation, somatosensation, and nociception) and associated limbic pathways. We then detail procedures for controlled solution delivery. For complete details on the use and execution of this protocol, please refer to Park and Lee.¹.