Targeting Collagens and LOX Through Plant-Derived MicroRNAs (XenomiRs)

Collagens and Lysyl Oxidases shape the extracellular matrix, with their dysregulation promoting matrix stiffness and pathological conditions. Plant-derived microRNAs (xenomiRs) can act across species, potentially modulating human physiology. Here, we present a robust protocol for isolating and sequencing xenomiRs from plants of the Evia region of Greece and predicting their human targets, highlighting their potential to regulate collagens and lysyl oxidases and providing a framework for cross-kingdom therapeutic approaches.