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Updated: Sep 3, 2026

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
Published on: January 7, 2020
Single-Cell and Single-Embryo RNA Sequencing
Bastien Ducreux1, Lucile Ferreux1,2, Julie Firmin1,2
1Université de Paris Cité, Faculty of Medicine, INSERM 1016, Paris, France.
Abstract:
The scarcity of available donated human embryos for research and technical difficulties present numerous challenges for human development research. Developments in RNA-seq technologies for small inputs, such as single embryos and single cells, now make human applications possible. This chapter outlines single-embryo RNA-seq, with adaptations noted for single-cell RNA-seq and single embryonic/abembryonic compartment applied to preimplantation embryo analysis, from embryo collection to bioinformatic data processing, and the specificities of applications of these techniques in reproductive biology.
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