Related Experiment Video

Updated: Sep 3, 2026

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells
09:46

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells

Published on: December 27, 2017

The Use of Bioengineered Induced Pluripotent Stem Cell-Derived Human Megakaryocytes to Diagnose and Characterize

Nanyan Zhang1, Peter J Newman2,3,4

  • 1Blood Research Institute, Versiti Blood Center of Wisconsin, Milwaukee, WI, USA. nzhang@versiti.org.

Methods in Molecular Biology (Clifton, N.J.)
|September 2, 2026
PubMed

Anti-platelet allo-, auto-, and iso-antibodies, which target platelet surface glycoproteins, play a crucial role in immune-mediated platelet destruction across various clinical scenarios. Whole cell platelet assays-while effective in maintaining the conformation of the offending antigen-are limited in characterizing anti-platelet antibodies due to their lack of specificity. Here, we describe a novel strategy for generating bioengineered HLA class I-negative, "antigen-specific" megakaryocytes (MKs) from induced pluripotent stem cells (iPSCs). These engineered cells can be cryopreserved for later distribution and analysis. Combining bioengineered megakaryocytes with flow cytometry allows for rapid detection and characterization of platelet-specific antibodies present in patient plasma. The enhanced sensitivity and remarkable specificity of this approach hold great promise for diagnosing a variety of immune platelet disorders.

Keywords:
Anti-platelet antibodyGene editingMegakaryocyteiPSC

More Related Videos

Leukodepletion Filters-Derived CD34+ Cells As a Cell Source to Study Megakaryocyte Differentiation and Platelet Formation06:57

Leukodepletion Filters-Derived CD34+ Cells As a Cell Source to Study Megakaryocyte Differentiation and Platelet Formation

Published on: May 20, 2021

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors14:30

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Published on: August 7, 2021

Related Concept Videos

EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...