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Updated: Sep 3, 2026

Production and Multi-Parameter Live Cell Fluorescence Lifetime Imaging Microscopy (FLIM) of Multicellular Spheroids
Published on: August 9, 2024
Spiro-helicenoid Carbofluoresceins: CPL-Active Emitters for Bioimaging
Laura Erviti-Marticorena1, Silvia Simón-Fuente2, Sandra Míguez-Lago1
1Department of Organic Chemistry, Faculty of Sciences, University of Granada, 18071Granada, Spain.
Abstract:
Chiral fluorescent probes based on small organic molecules present a promising platform for advanced bioimaging applications. In this work, we have developed a helically chiral spiro-shaped carbofluorescein with red emission (λem ≤ 613 nm). Configurationally stable enantiomers were separated, and electronic circular dichroism (ECD) and circularly polarized luminescence (CPL) evaluated. Preliminary results showed cellular permeability and intracellular emission.
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