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Updated: Sep 3, 2026

Isolation and Transcriptome Analysis of Plant Cell Types
Published on: April 7, 2023
Single-cell transcriptomics reveals a conserved GA-GASA1-ROS module that promotes lateral root founder cell
Xin-Qiao Du1, Rahul Shaw2, Jun Zhang1
1Shanghai Collaborative Innovation Center of Agri-Seeds, Joint Center for Single Cell Biology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Abstract:
The specification and commitment of lateral root founder cells (LRFCs) from postembryonic pericycle cells are critical steps in LR development, but their earliest molecular determinants remain unclear. Using single-cell transcriptomics, we resolved the transcriptional transitions that guide LRFC fate in Arabidopsis thaliana and identified five distinct phases of transcriptional progression during LRFC specification. Through analysis of differentially expressed genes and phenotypic observations, we showed that application of exogenous gibberellin (GA; 20 and 50 μM) promoted nuclear migration and division in LRFCs independently of DELLA signaling and revealed that gibberellic-acid-stimulated Arabidopsis 1 (GASA1) functions as a key mediator of GA to accelerate LRFC progression. Histochemical staining and cytological observations demonstrated that GASA1 mediates GA-triggered accumulation of reactive oxygen species (ROS) to reinforce LRFC commitment. The GA-GASA1-ROS module is evolutionarily conserved, and GA-ROS-driven LRFC commitment is fundamental across angiosperms. Notably, GASA1 emerged during root evolution in early land plants and diversified alongside lateral and shoot-borne root systems, revealing a conserved GA-ROS axis in plant organogenesis.
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