Flash-induced scattering transients in the 10 microseconds--5 s time range between 450 and 540 nm with Chlorella...

Flash-induced transients in light scattering were shown to occur with Chlorella cells. The kinetic and spectral patterns of the scattering transients and their relation to the absorption changes studied in the 10 microseconds--5 s time range, between 450 and 540 nm. 1. The kinetics of the fast changes (less than 500 ms) in scattering and absorbance were identical. From about 500 ms divergence of the two signals was observed. 2. The transient spectrum characterizing the fast scattering changes exhibited a large double band between 480 and 500 nm. Transients corresponding to the slower changes resembled the steady scattering spectrum (Latimer, P. and Rabinowitch, E. (1959) Arch. Biochem. Biophys. 84, 428--441) with a maximum at about 515 nm. 3. From theoretical considerations it is suggested that fast transients in scattering and absorbance are physically interrelated, and as has been shown for absorption changes (Witt, H.T. (1971) Q. Rev. Biophys. 4, 365--477) fast scattering transients can also be interpreted as an electrochromic phenomenon. Slower changes are accounted for by alterations in the microenvironment and conformation of the particles responsible for scattering.