Related Experiment Video
Updated: Sep 12, 2026

Introducing an Angle Adjustable Cutting Box for Analyzing Slice Shear Force in Meat
Published on: April 26, 2013
Thermal processing-induced proteolysis drives concurrent changes in bioactive peptides, flavor volatiles, and umami
Farouq Heidar Barido1, Kok-Gan Chan2, Abinawanto3
1Department of Biology, Faculty of Mathematics and Natural Science, University of Indonesia, Depok, West Java 16424, Indonesia; Department of Animal Science, Faculty of Animal Science, Universitas Sebelas Maret, Surakarta 57126, Indonesia; Food Technology of Animal Origin Research Group, Universitas Sebelas Maret, Surakarta 57126, Indonesia.
Abstract:
This study quantitatively profiled bioactive peptides, volatile organic compounds (VOCs), and umami nucleotides in dairy beef tenderloin subjected to five thermal processing methods (microwave, boiling, steaming, pan-grilling, oven) to elucidate their impacts on peptide bioactivity, flavor profiles, and sensory attributes. Gas chromatography-mass spectrometry identified 154 VOCs with method-specific clustering, pan-grilling maximized pleasant volatiles (pyrazines, aldehydes), total free amino acids (particularly sweet-tasting threonine, serine, alanine), and higher AMP nucleotides (p < 0.05), while peptidomics revealed elevated sarcoplasmic protein degradation yielding bioactive sequences, including DPP-IV and ACE-inhibitory peptides from enolase and creatine kinase. Sensory evaluation confirmed higher preferability in aroma, taste, and overall acceptability (p < 0.05) compared to that of steam cooking. These findings demonstrate pan-grilling optimizes flavor, sensory quality, and antihypertensive/antidiabetic peptide bioactivity to in dairy beef tenderloin.
Related Concept Videos
Pasteurization and Food Preservation
Microbes in Food Production
Methods of Controlling Food Spoilage
Protein Digestion
Bacterial Protein Maturation
Protein Denaturation

