FLInt in C. briggsae and C. tropicalis : Strains and resources for fast and locus-targeted integration of multi-copy transgenes
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Updated: Sep 12, 2026

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
Published on: March 16, 2012
Itai Antoine Toker1, Oliver Hobert1,2
1Department of Biological Sciences, Columbia University, New York, New York, USA.
Abstract:
C. briggsae and C. tropicalis are satellite model systems for molecular mechanistic investigations and for comparative studies across Caenorhabditis species. FLInt ( F luorescent L andmark Int erference) was developed in C. elegans for rapid generation of transgenic strains through integration of multi-copy DNA arrays into predetermined genomic loci, without requiring a random mutagenesis step. Here, we present resources and newly generated strains that enable FLInt in C. briggsae and C. tropicalis , yielding integrated transgenic lines within ~10 days of microinjection.

