Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Factors affecting competence for transformation in Staphylococcus aureus.

L Rudin, J E Sjöström, M Lindberg

    Journal of Bacteriology
    |April 1, 1974
    PubMed
    Summary

    Researchers developed a chemically defined medium for isolating amino acid-requiring mutants of Staphylococcus aureus. This medium optimizes bacterial transformation by identifying key factors like pH, temperature, and calcium ions for efficient genetic marker transfer.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The gene for staphylococcal protein A.

    Immunology today·2014
    Same author

    Single cell assay with an automated capillary microinjection system.

    Cytotechnology·2012
    Same author

    Purification of B. megatherium Phage G and Evidence for a Muralytic Enzyme as an Integral Part of the Phage.

    The Journal of general physiology·2009
    Same author

    The coxsackievirus and adenovirus receptor.

    Current topics in microbiology and immunology·2008
    Same author

    Preventive home visits to older people in Denmark--why, how, by whom, and when?

    Zeitschrift fur Gerontologie und Geriatrie·2007
    Same author

    Patch testing with methyldibromo glutaronitrile, a multicentre study within the EECDRG.

    Contact dermatitis·2005

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Bacterial Genetics

    Background:

    • Staphylococcus aureus is a significant human pathogen requiring genetic tools for research.
    • Understanding bacterial transformation is crucial for genetic manipulation and studying gene function.
    • Existing methods for isolating mutants and performing transformation assays have limitations.

    Purpose of the Study:

    • To develop a chemically defined medium for isolating amino acid-requiring mutants of Staphylococcus aureus strain 8325.
    • To optimize conditions for bacterial transformation assays using both plasmid and chromosomal markers.
    • To identify key factors influencing transformation efficiency in Staphylococcus aureus.

    Main Methods:

    • Development of a chemically defined growth medium.

    Related Experiment Videos

  • Systematic variation of pH, temperature, and cation concentrations (Ca ions) during transformation.
  • Assessment of transformation frequencies for various plasmid (erythromycin resistance) and chromosomal (trp, thy, cyt, tyr) markers.
  • Investigation of the role of phage phi11 lysogeny in transformation competence.

    • Main Results:

    • Optimal transformation pH is 6.75-7.0, and temperature is 30°C.
    • Calcium ions are essential for transformation; only phi11-lysogenic cells are transformable.
    • Maximal transformants achieved after 20 minutes of cell-DNA contact.
    • Similar transformation frequencies observed for plasmid ero and chromosomal trp, thy, cyt markers; tyr showed lower frequency.

    Conclusions:

    • A chemically defined medium effectively supports isolation of amino acid-requiring mutants and selective transformation in Staphylococcus aureus.
    • Transformation efficiency is significantly influenced by pH, temperature, calcium ions, and phage phi11 lysogeny.
    • The developed medium and optimized conditions provide a robust system for genetic studies of Staphylococcus aureus.