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Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
How molecular, supramolecular structure and gelatinization properties of starch influence fermentation behavior of
Yuanlong Chi1, Zongchen Li1, Ying Han2
1Innovation Center for Advanced Brewing Science and Technology, College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China.
Abstract:
The fermentation behavior of sorghum influences the distilled spirits quality, yet the differences among cultivars cannot be explained by amylopectin content alone. Six cultivars were studied to investigate how starch structure relates to fermentation. Fermentation stability was associated with supramolecular structure, correlating positively with single helix content (r = 0.81, p < 0.001), crystal heterogeneity (r = 0.62, p < 0.01), and retrograded crystal stability (r = 0.64, p < 0.01), which support sustained substrate release. The average fermentation rate was associated with gelatinization properties, correlating with amylose (r = 0.58, p < 0.05), short amylopectin chains (r = 0.74, p < 0.001), and long amylopectin chains (r = -0.74, p < 0.001). Principal component analysis revealed that gelatinization properties dominated PC1 (60.2%) and supramolecular structure dominated PC2 (23.2%), separating rapid, slow, and medium fermentation types. This study links starch structure to fermentation behavior, providing a basis for selecting sorghum cultivars for liquor production.
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