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Updated: Sep 21, 2026
3D Cell-Printed Hypoxic Cancer-on-a-Chip for Recapitulating Pathologic Progression of Solid Cancer
Published on: January 5, 2021
Protocol for paired isolation and characterization of migrating cancer cell subpopulations in 3D hypoxic
Luana Schito1, Sergio Rey-Keim1
1UCD School of Medicine and UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, D04 C7X2 Dublin, Ireland.
Abstract:
Paired isolation and characterization in 3D hypoxic microenvironments (HyPIC-3D) is a platform for delineating migratory heterogeneity among cancer cell subpopulations within 3D hypoxic tumor microenvironments. Here, we present the protocol for implementing HyPIC-3D using standard laboratory and computational resources. We describe the steps to perform migration assays with cancer spheroids, collect paired non-migrated and migrated subpopulations, and characterize their heterogeneous phenotypes through quantitative imaging, molecular, and cellular analyses. This protocol has potential for applications in diverse 3D migration contexts. For complete details on the use and execution of this protocol, please refer to Schito and Rey-Keim1.
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