Intestinal Stem Cells: Regulation, Plasticity, and Therapeutic Potential
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Updated: Sep 23, 2026

Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids
Published on: July 9, 2021
Samna Sagadevan1, Roei Cohen-Almagor1, Paloma Ordóñez-Morán2
1Translational Medical Sciences Unit, School of Medicine, Centre for Cancer Sciences, Biodiscovery Institute, University of Nottingham, Nottingham, UK.
Abstract:
Intestinal stem cells (ISCs) are central to the maintenance and regeneration of the gut epithelium, supporting its rapid turnover and adaptive capacity. Recent advances, including the identification of LGR5+ stem cells, 3D in vitro organoid technology, and single-cell transcriptomics, have uncovered the remarkable heterogeneity and plasticity of ISCs and their niche. The ISC niche comprises dynamic interactions among epithelial, stromal, immune, and microbial components, enabling responses to injury and disease. Notably, both active and reserve stem cell pools, as well as progenitor and differentiated cells, can contribute to regeneration. These insights are shaping novel strategies for treating gastrointestinal diseases and cancers through personalized medicine.
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