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Updated: Sep 30, 2026
Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
Published on: June 12, 2018
miRNA profiling identifies biomarkers and regulatory networks involving TGF-β/T-cell pathways in FPIES
Alberto Gómez-Carballa1, Julián Montoto-Louzao1, Sandra Viz-Lasheras1
1Unidade de Xenética, Instituto de Ciencias Forenses, Facultade de Medicina, Universidade de Santiago de Compostela, and Genética de Poblaciones en Biomedicina (GenPoB) Research Group, Instituto de Investigación Sanitaria (IDIS), 15706 Hospital Clínico Universitario de Santiago (SERGAS), Galicia, Spain; Genetics, Vaccines and Infections Research Group (GenViP), Instituto de Investigación Sanitaria de Santiago, 15706 Universidade de Santiago de Compostela, Santiago de Compostela, Galicia, Spain; Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBER-ES), Madrid, Spain.
Background:
Acute food protein-induced enterocolitis syndrome (FPIES) is a potentially life-threatening, non-IgE-mediated food allergy that primarily affects young children. Currently, diagnosis relies on clinical presentation and oral food challenges (OFC), which carry inherent risks. Consequently, FPIES is often misdiagnosed, leading to unnecessary interventions and delayed management. Despite its clinical relevance, the molecular mechanisms underlying FPIES remain poorly understood.
Objective:
To investigate the role of microRNAs (miRNAs) in FPIES and assess their diagnostic potential.
Methods:
We analyzed miRNAs expression profiles in peripheral blood from 29 children with OFC-confirmed moderate to severe FPIES reactions, collected on the OFC day at baseline (TP1), reaction onset (TP2), and four hours post-reaction onset (TP3). Using a hybrid ensemble feature selection approach, we compared these profiles to those of 26 children with acute gastroenteritis (AGE) and sepsis to identify FPIES-specific miRNA signatures.
Results:
Longitudinal analysis revealed an early heterogeneous miRNA response that evolved into a more consistent profile in TP3, suggesting a dynamic and evolving regulatory response. Eleven miRNAs emerged as key regulators, highlighting pathways partially shared with other allergic and inflammatory conditions. In silico pathway analysis identified TGF-β signaling and T-cell differentiation as candidate pathways potentially involved in FPIES. Importantly, we discovered and validated 26 miRNAs that accurately distinguish FPIES from sepsis and AGE, including a minimal 2-miRNA diagnostic signature (AUC= 0.98). miRNA-29a correlated with severe FPIES reactions, suggesting involvement in severity mechanisms.
Conclusion:
Our study reveals novel miRNA-mediated mechanisms in FPIES and identifies candidate biomarkers with diagnostic utility, paving the way for non-invasive molecular tools and therapeutic strategies.
