Related Experiment Video
Updated: Oct 3, 2026

Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy (VA-TIRFM)
Published on: October 2, 2012
Interferometry at the onset of total internal reflection
Abstract:
We report that nanometric axisymmetric surface deformations probed near the onset of total internal reflection produce a strongly anisotropic phase response. The local surface slope modifies the incidence angle, and the steep angular dependence of the Fresnel reflection phase near the critical angle converts these slope variations into measurable phase modulation. As a result, an axisymmetric bulge or dimple generates elliptical interference fringes aligned with the plane of incidence. When the deformation is sufficiently large, the local incidence angle falls below the critical angle, leading to a breakdown of total internal reflection and the appearance of a dark central region. Analytical relations linking the deformation profile, angular deviation, and the radius of the dark region are derived, enabling quantitative measurement of nanoscale surface deformation at fluid interfaces. This approach is useful for non-contact metrology of soft interfaces, thin-film mechanics, and biofluid diagnostics.
More Related Videos
06:43Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
Published on: May 3, 2022
16:10A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
Published on: March 22, 2012
Related Concept Videos
Total Internal Reflection Fluorescence Microscopy
Interference and Diffraction
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview
The ATR process begins by directing a beam...
Interference and Superposition of Waves
Interference occurs in mechanical waves, such as sound waves, waves on a string, and surface water waves. Mechanical waves correspond to the physical displacement of particles. Hence,...
IR Spectrometers
Reflection of Waves