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Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri
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Published on: September 23, 2017

Construction of Salmonella Transposon Mutant Library

Dan Gu1,2,3, Zhiming Pan4,5,6

  • 1Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu, China.

Methods in Molecular Biology (Clifton, N.J.)
|October 13, 2026
PubMed

Keywords:
SalmonellaFunctional genomicsGenome-wide mutagenesisTransposon mutant library

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DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...