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Updated: Oct 19, 2026
RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
Published on: March 11, 2014
Distinct epigenetic signatures define HPV-positive and HPV-negative tonsil carcinomas
M van der Heijden1, M Kommer2, J B Boers3
1Department of Head and Neck Surgery, Erasmus University Medical Center, Dr. Molewaterplein 40, Rotterdam, 3015 GD, The Netherlands; Department of Head and Neck Surgery and Oncology, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
Introduction:
Epigenetic modifications, particularly DNA methylation, play a key role in oropharyngeal squamous cell carcinoma (OPSCC). This is the first study to apply genome-wide methylated DNA sequencing (MeD-seq) to identify differentially methylated regions (DMRs) in OPSCC. By comparing HPV-positive and HPV-negative tonsillar carcinomas, we evaluate MeD-seq's applicability to validate known and identify novel DMRs. These findings may support the identification of potential biomarkers for risk stratification and prognostic assessment in OPSCC.
Methods:
Methylated DNA sequencing (MeD-seq) was used to analyze genome-wide DNA methylation patterns in formalin-fixed paraffin-embedded (FFPE) tonsil samples. Gene set enrichment analysis explored the biological role of DMR associated genes. A literature search of DMR's was performed to compare MeD-seq to other techniques.
Results:
Thirty-one analyzed samples (10 HPV + OPSCC, 9 HPV- OPSCC, and 12 controls) revealed 4,962 DMRs. We focused on the top five promoter-region DMRs with the highest fold change per comparison (HPV + vs. HPV-; HPV + vs. controls; HPV - vs. controls). Key DMRs involved IRF4, TCF24, and CLSTN2. IRF4 was hypermethylated in both OPSCC groups compared to controls, while CLSTN2 was hypermethylated in HPV + versus HPV - tumors. Enrichment analysis indicated enrichment of cell cycle-related pathways in HPV + tumors and IGF1R signaling pathways in HPV - tumors. MeD-seq confirmed known methylation patterns and identified novel OPSCC-specific DMRs.
Discussion:
This pilot study demonstrates HPV-associated methylation profiles in OPSCC, highlighting MeD-seq as a reliable technique for tumor methylation analysis, confirming existing and identifying novel DMRs. Larger studies are needed to validate potential biomarkers for prognosis and personalized treatment.
