Related Experiment Video
Updated: Oct 24, 2026

Technical Approach for Structural Analysis of an Unknown Compound in Huoxiang Zhengqi Oral Liquid based on Linear Ion Trap Mass Spectrometry
Published on: April 3, 2026
Analytical Insights into Biologically Active Substances Using Mass Spectrometric Techniques
Mihaela Silion1, Anca-Roxana Petrovici2, Ioana-Mirela Vasincu3
1Physics of Polymers and Polymeric Materials Department, "Petru Poni" Institute of Macromolecular Chemistry, 41A Grigore Ghica Voda Alley, 700487, Iasi, Romania. silion.mihaela@icmpp.ro.
Abstract:
Currently, research on biologically active substances with therapeutic properties is advancing along two main directions. Firstly, considerable efforts are being devoted to reducing side effects by incorporating these drugs into various matrices that play an essential role in controlled drug delivery and release. On the other hand, new compounds derived from existing active substances are being synthesized-compounds that are cheaper to obtain, display fewer adverse reactions, and simultaneously match the therapeutic efficacy of the drugs from which they originate. Moreover, one of the most effective techniques for analyzing these compounds continues to be HPLC-MS. Its high sensitivity for detecting extremely low concentrations (on the order of ng/mL), together with its precision, accuracy, reproducibility, and reasonable operating cost, makes HPLC--MS an indispensable tool in any research laboratory. This review aims to summarize recent advances in HPLC-MS methods for the detection and quantification of three classes of therapeutic substances (anti-inflammatory, anti-diabetic, and anti-cancer drugs) and to provide perspectives on their applications in pharmacokinetic studies, quality control, and drug metabolism research.
More Related Videos
08:16Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows
Published on: January 16, 2018
07:33Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
Published on: October 15, 2018
Related Concept Videos
Tandem Mass Spectrometry
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Mass Spectrometry: Overview
MALDI-TOF Mass Spectrometry
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Mass Spectrometers