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Purification and partial characterization of maize mosaic virus.
Intervirology
|January 1, 1979
Summary
Maize mosaic virus (MMV) purification revealed it contains single-stranded RNA and lipids. These findings support MMV
Area of Science:
- Plant Virology
- Molecular Biology
- Biochemistry
Background:
- Maize mosaic virus (MMV) is a significant plant pathogen affecting maize crops.
- Understanding the virus's structure and genetic material is crucial for developing control strategies.
Purpose of the Study:
- To purify and characterize the physical and chemical properties of maize mosaic virus (MMV).
- To determine the nature of the nucleic acid and other constituents within MMV particles.
Main Methods:
- Purification of MMV from infected maize leaf extracts using activated charcoal, Celite filtration, and sucrose density gradient centrifugation.
- Analysis of viral nucleic acid using orcinol and diphenylamine tests, and susceptibility to pancreatic RNase.
- Determination of buoyant density and lipid content.
Main Results:
- MMV particles were successfully purified, with a buoyant density of 1.181 g/ml in sucrose.
- The viral nucleic acid was identified as single-stranded RNA.
- Virus preparations were found to contain lipids.
Conclusions:
- The characterized morphology and constituents of MMV are consistent with those of a rhabdovirus.
- The findings provide essential data for classifying MMV and understanding its replication cycle.