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Updated: Oct 25, 2026

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
Published on: September 30, 2016
Identification and Cyclization Mechanism of Fungal (+)-β-Chamigrene Synthase
Yang Gao1, Li-Li Guo1, Subiy Akbar1
1School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan430074, China.
Abstract:
Chamigranes are a unique class of sesquiterpenoids featuring a spiro[5.5]undecane framework, yet their biosynthesis in fungi has remained elusive for decades. In this study, we identify and characterize ChamTS, a sesquiterpene synthase from the basidiomycetous fungus Cerrena albocinnamomea, which specifically catalyzes the formation of (+)-β-chamigrane. In vitro isotope-labeling experiments unveiled the cyclization pathway of ChamTS, revealing an initial 1,6-cyclization followed by a 1,5-proton shift and spirocycle-forming rearrangement. This work provides a foundation for the biosynthetic production and further engineering of β-chamigrane-type terpenoids.
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