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Updated: Oct 25, 2026

A Robotic Platform for High-throughput Protoplast Isolation and Transformation
Published on: September 27, 2016
Creating the first synthetic plastid genome in plants
Scott C Lenaghan1, Ralph Bock2, Daniel L Dunkelmann2
1Center for Agricultural Synthetic Biology (CASB), University of Tennessee Institute of Agriculture, Knoxville, TN 37996, USA; Department of Biochemistry & Cellular and Molecular Biology (BCMB), University of Tennessee, Knoxville, TN 37996, USA; Department of Food Science, University of Tennessee, Knoxville, TN 37996, USA.
Abstract:
Over the last decade, there has been a shift in biotechnology from genome reading (sequencing) to genome writing (engineering). While significant progress has been made in prokaryotes and yeast, genome writing remains in its infancy in plants. Unlike their complex nuclear genomes, the compact and biotechnologically attractive plastid genome represents an ideal target to launch plant genome writing at scale.
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