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Published on: December 5, 2020
Enhancing grapefruit resistance to green mold through synergistic effects of bamboo vinegar and Cryptococcus
Ruiqi Peng1, Han Ye1, Chunhua Wu2
1Forestry College, Southwest Forestry University, Kunming 650224, PR China.
Abstract:
Green mold (Penicillium spp.) is a major postharvest disease of grapefruit. Optimal concentrations of bamboo vinegar (BV) and Cryptococcus laurentii were screened in vitro, and their individual and combined efficacy evaluated. BV (1%, 121 °C-sterilized) plus 1 × 107 CFU mL-1 C. laurentii (BV-C. laurentii) significantly inhibited spore germination and germ tube elongation in Penicillium spp., disrupted P. digitatum hyphal cell wall and membrane integrity, and increased extracellular chitinase and β-1,3-glucanase activities by 1.05- and 1.22-fold over C. laurentii alone at 48 h while reducing malondialdehyde and reduced glutathione. BV-C. laurentii also activated fruit innate defense, with phenylalanine ammonia-lyase and polyphenol oxidase activities 1.03-1.44-fold higher than CK and total phenol and flavonoid contents 20.22% and 19.73% higher on day 28, without adversely affecting total soluble solids, titratable acidity, ascorbic acid or reducing sugar. Thus, BV-C. laurentii effectively controls green mold and delays quality deterioration in grapefruit.
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