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Updated: Oct 26, 2026
Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
Published on: February 1, 2019
Lipoplex Nanoparticle-Based RNA Delivery to 3D Melanoma Spheroids
Rüdiger Rudolf1,2, Tiziana Cesetti3,4, Florian Keller3,4
1CeMOS Research and Transfer Center, Technische Hochschule Mannheim, Paul-Wittsack-Straße 10, Mannheim, Germany. r.rudolf@th-mannheim.de.
Abstract:
Lipoplex nanoparticle-based vaccination technologies have shown tremendous efficiency for in vivo transfection during the COVID-19 pandemic and also hold promise for treating melanoma. While pharmacodynamics and pharmacokinetics need careful in vivo and clinical testing, other parameters of drug performance can be assessed with in vitro systems as well. Spheroids composed of more than one cell type can serve to assess drug penetration, cell-type specificity, and transfection efficiency at an early stage of drug development.
