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Updated: Oct 27, 2026
Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology
Published on: March 12, 2015
Ferrocene-Branched Polymers via a Ugi Multicomponent Reaction for an Integrated and Reagentless Amperometric Glucose
Jorge Urraca1, Isabel Zurita1, Marija Krznaric1
1Nanosensors and Nanomachines Group, Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040 Madrid, Spain.
Abstract:
A Ugi multicomponent reaction was employed to synthesize a ferrocene (Fc)-branched polyethyleneimine derivative. This polymer was evaluated as a redox-active matrix for the fabrication of an electroanalytical disposable glucose biosensor based on a carbon PE-printed electrode functionalized with glucose oxidase as a biorecognition element. The resulting biosensor displayed a linear response up to 35 mM glucose at pH 6.5, with a limit of detection of 33 µM. The enzyme biosensor reached 95% of the steady-state current within approximately 50 s and showed a sensitivity of 0.92 µA/mM. In addition, this analytical device exhibited high selectivity, good reproducibility, and excellent storage stability, retaining strong analytical performance after 45 days at 4 °C. The proposed electrochemical biosensor was successfully applied to glucose determination in red and white wine samples.
