The NEO-1 gene may serve as a potential biomarker for predicting acute myocardial infarction
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Zhaolin Yang1, Heyu Meng2, Jianjun Ruan1
1Department of Cardiology, China-Japan Union Hospital of Jilin University, Jilin University, Changchun, China.
Objectives:
This study elucidated the association between acute myocardial infarction (AMI) and Neogenin-1 (NEO-1) using proteomics to identify NEO-1 as a promising circulating biomarker for AMI risk prediction.
Methods:
A total of 107 participants were included in the analysis, comprising 45 control subjects without coronary heart disease (non-coronary heart disease [NCHD]; as a control group) and 62 patients with AMI. Furthermore, mRNA levels were elucidated via RT-qPCR, whereas the protein content was measured using enzyme-linked immunosorbent assay (ELISA). Lastly, the acquired data were elucidated and compared.
Results:
The data showed that relative to the NCHD group, the AMI group's NEO-1 mRNA level in peripheral blood (PB) mononuclear cells was 1.47 times higher (p<0.05), whereas NEO-1 protein levels were 1.50 times higher (p<0.05). Furthermore, in AMI patients, the expression of NEO-1 was not related to smoking, total cholesterol, alcohol consumption, triglycerides, age, high-density lipoprotein cholesterol, hypertension, or platelets. Moreover, Logistic regression assessment revealed that AMI was associated with elevated NEO-1 expression and enhanced fasting blood glucose.
Conclusions:
This study revealed that relative to the NCHD patients, AMI patients had elevated PB NEO-1 expression, which was an independent risk factor for AMI. These findings indicate that NEO-1 expression is a potential bio-index for AMI.
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