Effects of functionalized yeast hydrolysates as steeping media on nutritional properties and digestibility of germinated barley
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Updated: Nov 2, 2026

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
Elahe Abedi1, Seyed Mohammad Bagher Hashemi1, Shahrzad Maleki2
1Department of Food Science and Technology, Faculty of Agriculture, Fasa University, Fasa, Iran.
Abstract:
This study investigated the effects of germination combined with thiolated and/or aminated hydrolyzed yeast (HBSY) steeping solutions on the nutritional, structural, antioxidant, and biological properties of barley. Four treatments were evaluated: water-germinated barley (GW), thiolated HBSY (GS), aminated HBSY (GN), and combined thiolated/aminated HBSY (GSN). GSN showed the greatest enhancement in starch digestibility, exhibiting 52.1% rapidly digestible starch, ∼45% slowly digestible starch, and 2.7% resistant starch, along with improved protein digestibility (74.4%) and reduced trypsin inhibitor activity (3.12 TIU mg-1). SEM and SDS-PAGE analyses confirmed structural and protein profile modifications during germination. Phenolic enrichment and antioxidant capacity increased significantly, with TPC rising from 1170 to 5432 mg GAE/L and DPPH activity from 37.4% to 94.3%. Biological evaluations demonstrated acceptable cytocompatibility and hemocompatibility, with hemolysis remaining below 5%. Overall, GSN-treated barley exhibited improved functional properties, suggesting its potential as a value-added food ingredient.
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