In Vitro Myelination Using DRG Explants: An Efficient Model for Quantitative Evaluation of Peripheral Nerve Myelination in Culture
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Updated: Nov 2, 2026
In Vitro Myelination of Peripheral Axons in a Coculture of Rat Dorsal Root Ganglion Explants and Schwann Cells
Published on: February 10, 2023
Toshiyuki Araki1, Yurika Numata-Uematsu2, Shinji Tokunaga2
1Department of Peripheral Nervous System Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, 187-8502, Japan. taraki@ncnp.go.jp.
Abstract:
In vitro myelination, involving Schwann cells and dorsal root ganglion neurons, is an indispensable tool for studying the myelination process and evaluating the effects of various interventions under both physiological and pathological conditions in the peripheral nervous system. Here, we describe our DRG explant-based in vitro myelination (IVDME) culture method, which achieves more efficient myelination compared to conventional in vitro approaches.
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