Measuring Conduction Velocity by Compound Action Potential (CAP) Recordings
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Updated: Nov 2, 2026

Modeling Biological Membranes with Circuit Boards and Measuring Electrical Signals in Axons: Student Laboratory Exercises
Published on: January 18, 2011
Raphael P Ricci1, Carlie L Cullen2,3
1Mater Research Institute-The University of Queensland, Translational Research Institute, Woolloongabba, Queensland, Australia.
Abstract:
The recording of compound action potentials (CAPs)-the summed activity of multiple axons-is a reliable method for calculating the average conduction velocity of a population of axons. This chapter presents a detailed overview of how CAP recordings can be used to quantify action potential conduction velocity in acute brain slices of the adult mouse brain. We describe a step-by-step protocol for recording CAPs in the medial corpus callosum, highlighting technical considerations that improve data quality and reproducibility. Methods for analyzing CAP waveforms are discussed, including strategies to distinguish between fast-conducting myelinated fibers and slower unmyelinated populations. By providing practical guidance on experimental design, execution, and analysis, this chapter underscores the enduring value of CAP recordings as a versatile tool for probing axonal conduction and advancing our understanding of central nervous system function in health and disease.
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