[Purification of Salmonella abortus ovis H antigen by using immunoadsorption]

OH-antiserum was first produced in rabbit immunization with Salmonella abortus ovis cultures and was later used for obtaining an immunoadsorbent after the method of Avrameas and Thernynck. The raw (not purified) H-antigen produced after the method of McCoy et al., was purified by the immunoadsorbent and its activity and specificity were studied serologically by the reaction of Ouchterlony. It was established that the antigen reacts with the anti-H-salmonella serum, but does not react with normal sheep serum, which is an indication that it is purified and active. It was established also that immunoadsorption is a method suitable for H-antigen production from bacterial cilia.