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Novel bacteriophage lambda mutation affecting lambda head assembly.

C P Georgopoulos, R Bisig, M Magazin

    Journal of Virology
    |February 1, 1979
    PubMed
    Summary
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    A novel phage lambda mutation, dc10, disrupts head assembly, causing temperature sensitivity and altered host range. This mutation affects specific protein interactions during bacteriophage assembly.

    Area of Science:

    • Molecular Biology
    • Virology
    • Genetics

    Background:

    • Bacteriophage lambda is a model system for studying DNA packaging and head assembly.
    • Proper assembly of the phage head is crucial for viral infectivity and replication.
    • Understanding mutations affecting assembly provides insights into protein-protein interactions and structural requirements.

    Purpose of the Study:

    • To isolate and characterize a novel mutation in phage lambda affecting head assembly.
    • To elucidate the genetic and phenotypic properties of the dc10 mutation.
    • To investigate the impact of dc10 on the composition of aberrant phage heads.

    Main Methods:

    • Isolation and characterization of a novel phage lambda mutation (dc10).
    • Plaque assays at different temperatures and on specific host strains.

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  • Genetic mapping of the mutation to the B gene and dominance testing.
  • Analysis of phage head protein composition under different conditions.

    • Main Results:

    • The dc10 mutation confers temperature-sensitive plaque formation (viable at 42°C, not at 30°C or 37°C).
    • dc10-carrying phage exhibit host-specific defects, failing to form plaques on pN-constitutive hosts at 42°C.
    • The mutation maps to the B gene and is dominant over the wild-type allele.
    • At 30°C, dc10 leads to abnormal petit lambda heads containing pE, pB, pC, and pNu3.
    • Under pN-constitutive conditions, dc10 results in abnormal petit lambda heads composed of pE, X1, and X2.

    Conclusions:

    • The dc10 mutation specifically interferes with phage lambda head assembly.
    • The temperature sensitivity and host range phenotype are directly attributable to the dc10 mutation.
    • The data support a model explaining the role of the B gene product in head formation and assembly regulation.