Degradation of angiotensin II by plasmin

1. Synthetic angiotensin II (Hypertensin, Ciba, Basel) was incubated with a water-insoluble preparation of plasmin (E.C. 3.4.4.14) and the resulting products analysed by paper chromatography and N-terminal amino-acid analysis.2. Only the N-terminal asparagine was split off from the peptide. This indicates that plasmin attacks the Asn-Arg but not the Arg-Val in angiotensin II molecule.