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Related Experiment Videos

Interferon: evidence for its glycoprotein nature.

F Dorner, M Scriba, R Weil

    Proceedings of the National Academy of Sciences of the United States of America
    |July 1, 1973
    PubMed
    Summary

    Rabbit interferon is a glycoprotein, meaning it contains carbohydrates. This study confirms it has a specific sugar structure involving sialic acid and galactose.

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    Area of Science:

    • Biochemistry
    • Virology
    • Glycobiology

    Background:

    • Interferons are crucial antiviral proteins.
    • The structural composition of rabbit interferon, particularly the presence of carbohydrates, was previously unclear.

    Purpose of the Study:

    • To investigate the potential role of carbohydrates in the structure of rabbit interferon.
    • To elucidate the specific oligosaccharide sequence present in rabbit interferon.

    Main Methods:

    • Enzymatic treatment with neuraminidase and galactose oxidase.
    • Radiolabeling using tritiated sodium borohydride and N-\[(14)C\]acetylneuraminic acid.
    • Isoelectric focusing and affinity chromatography using phytohemagglutinin.

    Main Results:

    • Neuraminidase treatment resulted in homogeneous charge, indicating sialic acid removal.
    • Galactose oxidase treatment and reduction yielded active "asialointerferon".
    • Re-incorporation of N-\[(14)C\]acetylneuraminic acid restored charge heterogeneity and dual radiolabeling.
    • Asialointerferon binding to phytohemagglutinin confirmed the presence of terminal galactose.

    Conclusions:

    • Rabbit interferon is a glycoprotein.
    • It possesses a terminal oligosaccharide sequence of sialic acid followed by galactose.

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