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Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue
Published on: June 21, 2018
Direct measurement of proton binding to the active ternary complex of pig heart lactate dehydrogenase
The Biochemical Journal
|August 1, 1973
Abstract:
With the use of a glass electrode it is shown that one proton is taken up from the solvent when o-nitrophenylpyruvate forms a ternary complex with lactate dehydrogenase and NADH at pH8 and before the complex has rearranged to give products.
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