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Changes in membrane structure associated with cell contact.

R E Scott, L T Furcht, J H Kersey

    Proceedings of the National Academy of Sciences of the United States of America
    |December 1, 1973
    PubMed
    Summary
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    Cell-to-cell contact in 3T3 fibroblasts causes intramembranous particle aggregation, a change not seen in transformed cells. This suggests membrane protein redistribution influences cell proliferation control.

    Area of Science:

    • Cell Biology
    • Biophysics

    Background:

    • Cell membrane structure is crucial for cellular functions.
    • Intramembranous particles are key components of cell membranes.
    • Malignant transformation alters cell membrane characteristics.

    Purpose of the Study:

    • To investigate changes in 3T3 fibroblast cell membrane structure during cell-to-cell contact.
    • To compare membrane structure in normal and virally transformed cells.
    • To explore the relationship between membrane particle distribution and cell proliferation.

    Main Methods:

    • Ultrastructural analysis using freeze-cleavage technique.
    • Culturing of 3T3 fibroblasts at varying densities.
    • Observation of intramembranous particle distribution on membrane fracture faces.

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    Main Results:

    • Non-contacted 3T3 cells exhibit random intramembranous particle distribution.
    • Cell-to-cell contact induces significant aggregation of intramembranous particles in 3T3 cells.
    • Transformed SV3T3 and SP3T3 cells lack particle aggregation despite extensive cell contact.

    Conclusions:

    • Cell-to-cell contact in normal fibroblasts leads to redistribution of intramembranous particles.
    • Transformed cells display altered membrane particle organization.
    • Changes in membrane protein distribution associated with particle aggregation may regulate cell proliferation.