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Influence of stroma-free hemoglobin solution on renal function in dogs
Zeitschrift Fur Experimentelle Chirurgie
|January 1, 1979
Summary
Stroma-free hemoglobin solution (SFHS) effectively transported oxygen during kidney perfusion and blood exchange. However, hemoglobin casts formed in kidneys, particularly after isolated perfusion, due to potential stroma remnants or low urine pH.
Area of Science:
- Biomedical Engineering
- Physiology
- Nephrology
Background:
- Stroma-free hemoglobin solution (SFHS) is a potential acellular oxygen carrier.
- Its efficacy in physiological applications like organ perfusion and transfusion requires thorough evaluation.
Purpose of the Study:
- To assess the oxygen transport capabilities of SFHS during kidney perfusion and exchange transfusion.
- To investigate the potential for hemoglobin cast formation in renal glomeruli following SFHS administration.
Main Methods:
- Extracorporeal perfusion of kidneys using SFHS as a perfusate.
- Exchange transfusion procedures utilizing SFHS.
- Histological examination of kidney tissues to identify hemoglobin deposition.
- Monitoring of urine pH during procedures.
Main Results:
- SFHS demonstrated adequate oxygenation and transport during both extracorporeal kidney perfusion and after exchange transfusion.
- Histological analysis revealed hemoglobin casts in glomeruli, predominantly after isolated kidney perfusion.
- Fewer casts were observed after in situ perfusion and exchange transfusion.
- Potential causes for cast formation include residual stroma or low initial urine pH during perfusion.
Conclusions:
- SFHS is a viable oxygen carrier for kidney perfusion and exchange transfusion, showing effective oxygen delivery.
- The formation of hemoglobin casts is a notable side effect, particularly in isolated perfusion, necessitating further investigation into its precise etiology and mitigation strategies.